10 10 7 1 1b 49 3 5 7 1b 5 5 6d Protocols | Zhu Lab | Perelman School of Medicine at the University of Pennsylvania 5 5 5 4f 6e 1 5 9 5 5 57 5 5 9 9 44 49 5 9 cc 9 9 5 5 5 5 5 1c 11a 128 5 5 123 9 5 fb 106 5 19 145 5 5 5 9 c 1 7 5 2 1 1 1 5

5 1 1d

5 5 9 7

59 Skip to Main Content b

5 5 5 5 5 9 1a

30

3d

11 1 1 1 1 6

1 5 8 1 11 c 5 5 f 5 1 5 7

6 9 5c

2 9 3b

15 1 21

63

Site Navigation

87 1 15

65 26 Home a
48 Lab Members a
4a Publications a
42 Lab News a
40 Gallery a
44 Protocols 1f

1 13

f

6 8

1 7

13

33

b

9 5 2c

2c

51 b8

13

31

24

3f 37 Zhu Lab 1c 24

11 60

Researching Bacterial Pathogenesis

1c 1b

b

9 5 a

1 1 1 5 1 1 1 87

1c

3a

64 26 Home 12
39 Lab Members 12
3b Publications 12
33 Lab News 12
31 Gallery 12
35 Protocols 12
6b a

7

1 1 1 1 1 1 2b

5 5 9 5 9 29

1 9 9 9 2c

29

11 11 81 11 11 21 2c

19 3b Protocols

11 21 11 1 11 11 21 13

f

9 9 1 1 1 1 9 38

70

Frequently-used, lab-tailored protocols. Penn login is required.

11 11 39

15 29 31
19 3b
40 60 Electro-Competent cells 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 63 Small volume Western blot 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 5a Single step b-gal 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 65 BACTH (Bacterial Two Hybrid) 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 4f MuGent 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 6f Arbitrary PCR_for pSC123, pNJ17, pTGL3 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 62 Rapid Vc electrocompetent 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 74 glycerolmannitol-electrocompetent cell prep 3a
19 29 1b
15 49
50 64 24 1d
2a
15 29 31
19 3b
40 7a Standard infant mouse competition protocol for Vc 3a
19 29 1b
15 49
50 64 24 1d
2a

9 5 1 1 1 8 5 9 5 5 5 5 d 9 5 5 c

5 5 5 5 5 7

1 1 9 1 1 7

1 1 4a 1 1 60 15b 1 1 111 1 1 1 45 1 8 7 0